Visit All-Acad.com with more than 100,000 Jobs for Academics!
                    
Position: Postdoctoral Research Fellow - Genome Architecture Imaging
Institution: Karolinska Institutet
Location: Solna, Stockholm, Sweden
Duties: A postdoctoral position is now available in the Laboratories for Quantitative Genome Biology to investigate different aspects of genome organization in healthy and disease conditions using advanced single-molecule microscopy techniques. The main goal of the project is to obtain a comprehensive atlas of chromosome shapes, locations, and the associated transcriptional activity at single-cell resolution, in different model systems. The successful candidate will primarily rely on our DNA and RNA FISH methodologies to generate large image datasets that will be analysed using state-of-the-art image analysis pipelines already implemented in our lab
Requirements: A clearly demonstrated prior experience in microscopy methods and previous work in the field of genome organization is mandatory. Passion for meticulous work, attention to detail, and protocol optimization and troubleshooting is necessary. A strong motivation to work in a highly interdisciplinary and collaborative environment, and willingness to interact with scientists with computational background is required. Experimentalists with a strong motivation to work alongside theoreticians and to learn analytical and programming skills are strongly encouraged to apply
   
Text: Login and apply English Swedish Norwegian Danish Finnish German Dutch Chinese (BETA) Polish French (BETA) Postdoctoral research fellow in Genome Architecture Imaging Login and apply Karolinska Institutet, Institutionen för Medicinsk biokemi och biofysik, Translational Medicine and Chemical Biology A postdoctoral position is now available in the Bienko and Crosetto Laboratories for Quantitative Genome Biology ( https://bienkocrosettolabs.org/ ) to investigate different aspects of genome organization in healthy and disease conditions using advanced single-molecule microscopy techniques. Research environment The Bienko and Crosetto groups are two highly integrated research groups working on genome architecture (Bienko group) and genome fragility (Crosetto group) in different contexts. We develop and apply quantitative microscopy and sequencing techniques, combined with advanced computational approaches, to investigate the link between genome architecture and fragility, and its functional consequences, in healthy and perturbed conditions, such as cancer and aging. We pioneered BLESS, the first method for direct genome-wide localization of DNA double-strand-breaks DSBs (Crosetto et al., Nature Methods 2013), and recently further advanced the technology by developing BLISS (Yan et al., Nat Commun 2017; Mirzazadeh et al., Meth Mol Biol 2018). We have also developed GPSeq, a new method based on massively parallel sequencing that enables to measure the radial nuclear position of thousands of genomic loci simultaneously (manuscript in preparation). Recently, we have established a high-throughput platform for high-resolution DNA and RNA fluorescence in situ hybridization (FISH), which enables us to probe the nuclear location and the transcriptional activity of hundreds of genomic loci in thousands of single cells. Our labs are located at the Science for Life Laboratory (SciLifeLab) situated at the Karolinska Institute campus in Stockholm. SciLifeLab is an interdisciplinary center for molecular biosciences with focus on health and environmental research, bringing under the same roof groups from four universities: Karolinska Institutet, KTH Royal Institute of Technology, Stockholm University and Uppsala University. The center features state-of-the-art technology platforms, including next-generation sequencing, high-throughput histology, super-resolution microscopy, proteomics, image analysis, and bioinformatics. The successful candidate will join an interdisciplinary and dynamic team of international researchers, including clinicians, biologists, biotechnologists, engineers, computer scientists, and physicists. Our mission is to transform the way we understand complex biological phenomena and diseases such as cancer, by integrating next-generation sequencing technologies, single-molecule microscopy methods, and advanced computational tools. Duties The main goal of the project is to obtain a comprehensive atlas of chromosome shapes, locations, and the associated transcriptional activity at single-cell resolution, in different model systems. The successful candidate will primarily rely on our DNA and RNA FISH methodologies to generate large image datasets that will be analysed using state-of-the-art image analysis pipelines already implemented in our lab. The successful candidate will closely interact with researchers applying sequencing-based methods to the same model systems, as well as with expert image analysts to process the high volume of image data generated. Specific tasks include: Cell culture and preparation of samples for DNA and RNA FISH Preparation of oligonucleotide probes using liquid handling robotics Performing DNA and RNA FISH and imaging the samples Image processing and analysis in synergy with a dedicated image analyst Entry requirements A person is eligible for a position as postdoctoral research fellow if he or she has obtained a PhD no more than seven years before the last date of employment as postdoc. A clearly demonstrated prior experience in microscopy methods and previous work in the field of genome organization is mandatory. Prior experience with FISH techniques is a strong advantage. Passion for meticulous work, attention to detail, and protocol optimization and troubleshooting is necessary. A strong motivation to work in a highly interdisciplinary and collaborative environment, and willingness to interact with scientists with computational background is required. Experimentalists with a strong motivation to work alongside theoreticians and to learn analytical and programming skills are strongly encouraged to apply. Application process An employment application must contain the following documents in English: A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work A complete list of publications A summary of current work (no more than one page) Verifications for crediting of illness, military service, work for labour unions or student organisations, parental leave or similar circumstances Verification from the thesis defence committee or the equivalent (only if the thesis defence is scheduled within three months after the application deadline) The application is to be submitted on the MyNetwork recruitment system. Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine. Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published. Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone. For temp agencies and recruiters, and to salespersons: We politely, yet firmly, decline direct contact with temp agencies and recruiters, as well as those selling additional job announcements. Type of employment Temporary position longer than 6 months Contract type Full time Number of positions 1 Working hours 100 % City Stockholm County Stockholms län Country Sweden Reference number 2-2556/2018 Contact Magda Bienko, 46 (0)72 857 29 25 Union representative Anne Edgren, OFR/S, P, O, 46 (0)8-616 16 29 Biborka Bereczky Veress, SACO, 46 (0)70-173 85 75 Henry Wölling, SEKO, 46 (0)8-524 800 00 Published 03.May.2018 Last application date 31.Aug.2018 11:59 PM CET Share links Login and apply Return to job vacancies Varbi Recruit recruitment system COOKIES Conditions and GDPR × Modal title
Please click here, if the Job didn't load correctly.







Please wait. You are being redirected to the Job in 3 seconds.