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Position: Research Assistant - Molecular and Cell Biology Laboratory
Institution: Salk Institute for Biological Studies
Location: La Jolla, California, United States
Duties: The Research Assistant position is part of a small team to establish and characterize stem cell-derived models of neurodegenerative disease (i.e. Alzheimer’s disease) and contribute to systematic, high-throughput experiments designed to identify new candidate genes relevant in disease pathology. Responsibilities include executing high-throughput lentiviral genome-wide CRISPR/Cas9 screens, including virus production and transduction of cultured stem cells, neurons and astrocytes. Cell line, primary cell, and stem cell culture; molecular biology and high-throughput screening
Requirements: Bachelor’s degree in a life science related field. Minimum 1 years of research experience. Significant experience with mammalian tissue culture
   
Text: Job #: F693 Posted Date: August 7, 2017 Closes On: Open Until Filled Job Title: Research Assistant I- Molecular and Cell Biology Laboratory, Dr. Patrick David Hsu Status: Regular Full Time Benefits Eligible: Yes Laboratory/Department: Molecular and Cell Biology Laboratory Laboratory research takes place at the intersection of bioengineering, genomics, and neuroscience to develop novel genome engineering technologies and uncover biological mechanisms behind neurodegenerative diseases and cancer. Position Description: The Research Assistant position is part of a small team to establish and characterize stem cell-derived models of neurodegenerative disease (i.e. Alzheimer’s disease) and contribute to systematic, high-throughput experiments designed to identify new candidate genes relevant in disease pathology. Responsibilities include executing high-throughput lentiviral genome-wide CRISPR/Cas9 screens, including virus production and transduction of cultured stem cells, neurons and astrocytes. Cell line, primary cell, and stem cell culture; molecular biology and high-throughput screening. Education: Required: Bachelor’s degree in a life science related field. Experience: Required: Minimum 1 years of research experience. Significant experience with mammalian tissue culture. Preferred: Prior experience with sensitive mammalian cells including stem cells or primary cells. Experience with molecular biology. Examples: PCR, plasmid purification, genomic DNA purification, RNA purification, qPCR. Neuroscience and/or genetics background. Special Requirements: Must be willing to work an adjusted schedule to support research needs. Must be willing to work in an animal-related research environment. Must be willing to work with human embryonic stem cells. Successful completion of the Institute’s background investigation. Physical Requirements: This position requires constant reasoning, analyzing and working inside.
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